C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/65 (2006.01) C12N 15/70 (2006.01)
Patent
CA 2694537
The present invention relates to a shuttle BAC vector for facilitating the cloning, transfer and het-erologous expression of streptomycete secondary metabolite biosynthetic gene clusters. The invention also relates to a plasmid rescue method using this vector for enhancing the process of cloning biosynthetic gene clusters for secondary metabolites from streptomycetes without sophisticated generation and screening of cosmids or BAC libraries. The cloned DNA can then be used for sequencing or heterologous expression of putative secondary metabolic gene clusters.
La présente invention porte sur un vecteur BAC navette pour faciliter le clonage, le transfert et l'expression hétérologue de batteries de gènes biosynthétiques métabolites secondaires de streptomycètes. L'invention porte également sur un procédé de sauvetage de plasmide utilisant ce vecteur pour améliorer le procédé de clonage de batteries de gènes biosynthétiques pour des métabolites secondaires à partir de streptomycètes sans génération et criblage sophistiqués de cosmides ou de bibliothèques de BAC. L'ADN cloné peut ensuite être utilisé pour le séquençage ou l'expression hétérologue de batteries de gène métaboliques secondaires putatifs.
He Min
Liu Hongbo
Torys Llp
Wyeth Llc
LandOfFree
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