Vectors and methods for the mutagenesis of mammalian genes

C - Chemistry – Metallurgy – 12 – N

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C12N 5/16 (2006.01) C12N 5/18 (2006.01) C12N 15/10 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12N 15/867 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2276529

Disclosed herein are methods for mutagenizing a mammalian gene, the methods involving introducing into a mammalian cell a retroviral vector which includes a splice acceptor sequence, a transcription termination sequence, and retroviral packaging and integration sequences, the introducing step being carried out under conditions which allow the vector to integrate into the genome of the cell. Also disclosed are retroviral vectors for use in these methods as well as methods for the use of mutagenized cells.

L'invention concerne des procédés permettant de mutagéniser un gène mammalien, consistant à introduire dans une cellule mammalienne un vecteur rétroviral, lequel comprend une séquence d'accepteur d'épissure, une séquence de terminaison de transcription et des séquence d'intégration et d'encapsidation rétrovirales, la première étape étant réalisée dans des conditions permettant d'intégrer le vecteur au génome de la cellule. L'invention concerne également des vecteurs rétroviraux destinés à être utilisés dans ces procédés, ainsi que des procédés d'utilisation de cellules mutagénisées.

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