Vectors, compounds and method for expression of a human...

C - Chemistry – Metallurgy – 12 – N

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530/13, 195/1.22

C12N 15/12 (2006.01) C07K 14/705 (2006.01) C07K 14/82 (2006.01)

Patent

CA 1340221

The present invention comprises novel recombinant DNA compounds which encode the ~40,000 dalton adenocarcinoma antigen recognized by monoclonal antibody KS 1/4. Eukaryotic and prokaryotic expression vectors have been constructed that comprise novel KSA-encoding DNA and drive expression of KSA when transformed into an appropriate host cell. The novel expression vectors can be used to produce KSA derivatives, such as non-glycosylated KSA, and to produce KSA precursors, such as nascent KSA, and to produce subfragments of KSA. The recombinant-produced KSA is useful for the diagnosis, prognosis and treatment of disease states including adenocarcinomas of the lung, prostate, breast, ovary and colon/rectum; and for the creation of novel antibodies for treatment or diagnosis of the above.

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