Vectors for multiple gene expression

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01)

Patent

CA 2686772

The present invention provides a vector for expressing at least a first and a second nucleic acid molecules which exhibit a percentage of homology of approximately 80% or greater than 80% over a portion of 40 or more continuous nucleotides and wherein said first nucleic acid molecule and/or said second nucleic acid molecule is modified so as to reduce said percentage of homology to less than 75%. The present invention also relates to substantially isolated nucleic acid molecules comprising a nucleotide sequence as defined in any of SEQ ID NO: 9-15 and 66-69. It also provides a host cell and a pharmaceutical composition comprising such a nucleic acid molecule or vector as well as their use for therapeutic or preventive purposes.

La présente invention concerne un vecteur d'expression d'au moins une première et une deuxième molécule d'acide nucléique qui présentent un pourcentage d'homologie d'environ 80 % ou de plus de 80 % sur une partie de 40 ou plus des nucléotides continus, la première molécule d'acide nucléique et/ou la deuxième molécule d'acide nucléique étant modifiée de façon à réduire ce pourcentage au-dessous de 75 %. La présente invention concerne également des molécules isolées d'acide nucléique comprenant les séquences nucléotidiques telles que définies dans un quelconque des numéros de séquence suivants: SEQ ID NO: 9-15 et 66-69. L'invention concerne également une cellule hôte et une composition pharmaceutique comprenant une telle molécule d'acide nucléique ou vecteur, ainsi que leur utilisation à des fins thérapeutiques ou préventives.

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