Vegetative growth-specific promoter and genetically modified...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2437203

DNA having a promoter activity for specifically expressing a foreign gene in a vegetative growth tissue (in particular, stem and/or leaf). This DNA is a rice 0sGA3ox2 gene promoter having the sequence represented by SEQ ID NO:1 or a sequence containing a part thereof and having a promoter activity comparable to the activity of the sequence of SEQ ID NO:1. An expression vector containing this promoter and a foreign gene ligated thereto in such a manner as enabling the expression; plant cells transformed by this expression vector; plants regenerated from these plant cells; offsprings and propagations of the plants; seeds obtained from these offsprings; and a method of transferring a foreign gene into a plant by using the above expression vector.

ADN exerçant une activité de promoteur servant à exprimer de façon spécifique un gène étranger dans un tissu de croissance végétative (en particulier, tige et/ou feuille). Cet ADN consiste en un promoteur de gène du riz OsGA3ox2 dont la séquence est représentée par SEQ ID NO:1 ou dont la séquence contient une partie de cette dernière et exerce une activité de promoteur comparable à l'activité de la séquence de SEQ ID NO:1. Vecteur d'expression contenant ce promoteur et gène étranger relié au dit vecteur de manière à favoriser l'expression ; cellules de plantes transformées par ce vecteur d'expression ; plantes régénérées à partir de ces cellules de plantes ; pousses et propagations de ces plantes ; semences obtenues à partir de ces pousses ; procédé de transfert d'un gène étranger afin de l'introduire dans une plante au moyen de ce vecteur d'expression.

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