Venting and drying of samples in multi-well plates

B - Operations – Transporting – 01 – L

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B01L 3/00 (2006.01)

Patent

CA 2497965

Methods for venting or drying (e.g., lyophilizing) samples contained in a multi-well plate. One method involves providing a multi-well plate that includes wells that each contain a sample and define an opening substantially sealed with a filter element that permits permeation of at least one gas and substantially prevents permeation of microbes. Another variant includes introducing a sample into a well via an opening defined by the well, covering at least a portion of the well opening with a filter element, and permitting gas to enter or exit the well substantially only through the filter element. An assembly is also described that includes a multi-well plate and a sheet defining a plurality of caps, wherein each of the caps includes a microbe- impermeable filter element. Another multi-well plate assembly includes a filter positioned to extend across at least a portion of the well opening.

L'invention concerne des procédés permettant d'aérer et de sécher (par exemple, de lyophiliser) des échantillons contenus dans une plaque à cupules multiples. Un procédé de l'invention consiste à utiliser une plaque à cupules multiples, chaque cupule comprenant un échantillon et définissant une ouverture sensiblement obturée par un élément filtre qui permet de laisser passer au moins un gaz et qui empêche sensiblement de laisser passer les microbes. En variante, un procédé consiste à introduire un échantillon dans une cupule par l'intermédiaire d'une ouverture définie par la cupule; à recouvrir au moins une partie de l'ouverture de cupule par un élément filtre; et à laisser un gaz pénétrer ou sortir de la cupule sensiblement seulement par l'élément filtre. L'invention concerne également un ensemble qui comprend une plaque à cupules multiples et une feuille définissant une pluralité de coiffes, chaque coiffe comprenant un élément filtre ne laissant pas passer les microbes. Un autre ensemble plaque à cupules multiples comprend un filtre positionné de façon à s'étendre sur toute la surface d'au moins une partie de l'ouverture de cupule.

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