C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/071 (2010.01) A61K 39/00 (2006.01) C12N 7/00 (2006.01)
Patent
CA 2624544
The invention relates to a Vero cell line which can grow in a culture free of serum and proteins, in suspension, in the absence of support materials for the adhesion thereof and to the production of viral vaccines using said cell line. More specifically, the invention relates to the establishment of a cell line which can grow in a suspension culture without said cells having to adhere to a support material. The invention also relates to a method of obtaining the Vero cell line and to a method of producing viral vaccines with said cell line. The invention further relates to the viruses obtained using the inventive method and to the vaccines formulated with said viruses.
L'invention porte sur une lignée cellulaire Vero convenant à une croissance dans une culture exempte de sérum et de protéines, en l'absence de matériaux de support pour son adhérence, et sur la production de vaccins viraux utilisant ladite lignée cellulaire. Plus particulièrement, l'invention porte sur l'établissement d'une lignée cellulaire qui peut croître dans une culture en suspension sans qu'il soit nécessaire que lesdites cellules adhèrent à un matériau de support. L'invention concerne également un procédé d'obtention de ladite lignée cellulaire Vero et un procédé de production de vaccins viraux avec ladite lignée cellulaire. L'invention concerne également les virus obtenus par le biais de ce procédé ainsi que les vaccins formulés avec lesdits virus.
Daelli Marcelo Gustavo
Etcheverrigaray Marina
Forno Angela Guillermina
Kratje Ricardo
Paillet Cristian
Mcmillan Llp
Zelltek S.a.
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