C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/28 (2006.01) C12Q 1/04 (2006.01)
Patent
CA 2065401
A very rapid method for the detection of a peroxidase-containing fungus comprises the steps of: (a) contacting a sample suspected of containing such fungus with a mixture which comprises (t) a peroxide selected from hydrogen peroxide and addition compounds thereof containing latent hydrogen peroxide; (ii) a scavenger (such as EDTA or a salt thereof) effective to remove heavy metals which may potentially decompose said peroxide catalytically; (iii) an alkaline buffer solution having the capacity to maintain the pH at a value within the range of 9.5 to 14; and (iv) an oxidizable substrate which comprises at least one compound selected from .beta.-(3,4-dihydroxyphenyl)-u-alanine (DOPA), caffeic acid and salts thereof excepting heavy metal salts; and (b) ob- serving whether within two hours from the contacting step an intense color develops indicating the presence of peroxidase-con- taining fungus in the sample. Also included is a test kit for carrying out the method of the invention.
Bar-Or David
Solomons Clive
Diagnostic Markers Inc.
Robic
LandOfFree
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