Vip54 protein and related materials

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2387188

"VIP54" protein was identified by yeast two-hybrid screening using H.pylori VacA as bait. VIP54 is not detected in neurons, but is detected in neuroblastoma cells removed from a tumour. Furthermore, VIP54 co-distributes with vimentin and decorates intermediate filaments with a dotted pattern. The invention provides proteins comprising the amino acid sequence of VIP54, as well as related peptides, nucleic acid and antibodies. These are useful as diagnostic reagents for neuroblastoma, as markers for intermediate filaments, and for the inhibition of the VIP54/VacA interaction.

Selon l'invention, on a identifié la protéine VIP54 par le criblage de levure à deux hybrides en utilisant en tant qu'appât VacA de H. pylori. VIP54 n'est pas détectée dans les neurones mais se détecte dans les cellules de neuroblastome extraites d'une tumeur. En outre, la VIP54 est co-distribuée avec la vimentine et décore les filaments intermédiaires avec un ornement à points. L'invention concerne aussi une protéine comprenant la séquence d'acides aminés de VIP54 ainsi que des peptides, des acides nucléiques et des anticorps y relatifs, qui s'utilisent en tant que réactifs diagnostiques du neuroblastome, comme marqueurs pour filaments intermédiaires et pour inhiber l'interaction VIP54/VacA.

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