Viral agents

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 35/76 (2006.01) C07K 14/075 (2006.01) C12N 7/00 (2006.01) C12N 15/34 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2361055

A viral DNA construct, and virus encoded thereby, is provided having one or more tumour specific transcription factor binding sites in place of one or more wild type transcription factor binding sites operatively positioned in the promoter region which controls expression of early genes responsible for viral nucleic acid replication. Preferred constructs place the tumour specific transcription factor binding sites in operative relation to DNA polymerase, DNA terminal protein and/or DNA binding protein. Compositions and constructs contained therein are provided, particularly for use in therapy. Methods of treating patients for neoplasms are also provided.

L'invention concerne une construction d'ADN viral et un virus codé par celle-ci, ladite construction possédant un ou plusieurs sites de combinaison avec le facteur de transcription spécifique des tumeurs à la place d'un ou plusieurs sites de combinaison avec le facteur de transcription du type sauvage, positionnés activement dans la région promotrice qui régule l'expression des gènes précoces responsables de la réplication d'acide nucléique viral. Les constructions préférées mettent les sites de combinaison avec le facteur de transcription spécifique des tumeurs en relation active avec l'ADN polymérase, la protéine terminale d'ADN et la protéine de liaison à l'ADN. Des compositions et des constructions, notamment à utiliser en thérapie, sont décrites ainsi que des méthodes de traitement des néoplasmes chez des patients.

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