Viral particles containing an alphavirus-derived vector and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 7/01 (2006.01)

Patent

CA 2547922

Viral particle consisting of structural elements not derived from an alphavirus and containing an alphavirus-derived vector made replication- defective by deletion, or replacement with at least one transgene, of the structural genes, characterized in that the structural elements of said particle are not encoded by the genome of the alphavirus-derived vector. Method for producing said particle, which consists in expressing in trans, in a cell line, the genes encoding the structural elements not derived from the alphavirus and the alphavirus-derived vector, and then in recovering the viral particles present in the cell culture supernatant.

Particule virale constituée d'éléments structuraux non issus d'un alpha-virus et contenant un vecteur dérivé d'alpha-virus rendu défectif pour la réplication, par délétion ou remplacement par au moins un transgene, des gènes structuraux caractérisée en ce que les éléments structuraux de ladite particule ne sont pas codés par le génome du vecteur dérivé d'alpha-virus. procédé de production de ladite particule qui consiste A exprimer en <i>trans</i>, dans une lignée cellulaire, les gènes codant les éléments structuraux non issus de l'alpha-virus et le vecteur dérivé d'alpha-virus puis à récupérer les particules virales présentes dans le surnageant de la culture cellulaire.

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