Virus-based amplification vector for plants

Details Virus-based amplification vector for plants Virus-based amplification vector for plants

C - Chemistry, Metallurgy

12

N

C12N 15/83 (2006.01) C12N 15/12 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2421306

The invention describes virus-based amplification vectors for plants containing additional plant-specific internal ribosome entry site (IRES) element(s) allowing for a polycistronic translation and a cap-independent translation of : a) heterologous gene(s); b) whole viral genome or c) viral subgenomic RNAs. Said IRES elements are of plant viral origin, or they are isolated from other organisms or engineered using different synthesis procedures. Said IRES element(s) and said heterologous gene(s) are inserted into ampification vectors and allow for the expression of said heterologous gene(s) in the absence of additional viral promoters, in particular, said expression is achieved through cap-independent translation.

L'invention concerne des vecteurs d'amplification à base de virus pour des plantes contenant un ou plusieurs sites d'entrée internes des ribosomes (IRES) supplémentaires spécifiques aux plantes, permettant une traduction polycistronique et une traduction indépendante de la coiffe a) d'un ou de plusieurs gènes, b) du génome viral complet ou c) des ARN viraux sous-génomiques. Ces éléments IRES sont obtenus à partir de virus de plantes, isolés à partir d'autres organismes ou mis au point à l'aide de différents processus de synthèse. Ces éléments IRES et lesdits gènes hétérologues sont introduits dans des vecteurs d'amplification et permettent l'expression desdits gènes hétérologues en l'absence de promoteurs viraux supplémentaires. Plus particulièrement, ladite expression est obtenue par traduction indépendante de la coiffe.

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