Virus preparations and methods

A - Human Necessities – 61 – K

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Details

A61K 39/245 (2006.01) A61K 35/76 (2006.01) A61P 31/20 (2006.01) C12N 7/02 (2006.01)

Patent

CA 2592141

Methods are disclosed for the preparation of herpesvirus, such as herpes simplex virus type 2 for vaccine use. Such viruses can be grown on serum free or serum containing media and can be prepared from the virus containing culture supernatant or virus containing cells. The virus is prepared for subsequent pharmaceutical formulation by methods which may include treatment with solid phase affinity reagents containing sulfate- or sulfonate-comprising binding groups. Such sulfated polysaccharide groups as heparin or dextran sulfate may be used, and eluted with salt solutions. The process can be combined with other culture, harvesting and formulation steps.

La présente invention décrit des méthodes de préparation d'herpèsvirus, tels que le virus herpès simplex de type 2, pour des applications de type vaccins. De tels virus peuvent être cultivés dans des milieux contenant ou non du sérum, et peuvent être préparés à partir du surnageant du milieu de culture contenant les virus, ou des cellules contenant les virus. Le virus est préparé afin d'être incorporé dans des formules pharmaceutiques par des méthodes pouvant inclure le traitement par des réactifs d'affinité en phase solide portant des groupements fonctionnels chélatants de type sulfate ou sulfonate. Des groupements polysaccharides portant des sulfates, tels que le sulfate d'héparine ou le sulfate de dextrane, peuvent être employés, et élués par des solutions salines. Ledit procédé peut être associé à d'autres étapes de culture, de récolte et de formulation.

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