G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/545 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2105515
ABSTRACT Visual Immunoassay Method For The Detection Of Ligands, Based On The Use Of Opaque Plastic Supports The present invention is related with the field of Immunology, and particulary with an immunoassay method for ligand detection in fluid bodies, using plastic supports. The technical objective of the invention consists of a manual immunoassay method that provides high sensitivity and specificity, and reagent economy, for the detection of ligands in fluid bodies, using opaque white plastic supports. The latter contain either antigens or antibodies, immobilized on low- depth microwells. The detection of the respective ligand binding partner (antibodies or antigens, respectively) is evidenced using reagents conjugated with monodisperse colloidal gold, being the signal amplified "in situ" by physical developers, based on silver ions. The samples, and all liquid reagents are placed in contact with the microwells, finally obtaining metallic colored insoluble reactions of very high contrast, that can be easily interpreted in a visual fashion. This method can be employed for the detection of any type of antigen (including small molecules with discreet epitope structure) or antibody.
de La C. Lescaille Buitrago Lissett
Santizo Lescaille Carlos A.
Centro de Ingieneria Genetica Y. Biotecnologia
Macrae & Co.
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