C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/34 (2006.01) G01N 15/14 (2006.01)
Patent
CA 2411465
The present invention provides methods and devices for the knowledge-based discovery and optimization of differences between cell types. In particular, the present invention provides visual servoing optical microscopy, as well as analysis methods. The present invention provides means for the close monitoring of hundreds of individual, living cells over time: quantification of dynamic physiological responses in multiple channels; real-time digital image segmentation and analysis; intelligent, repetitive computer-applied cell stress and cell stimulation; and the ability to return to the same field of cells for long-term studies and observation. The present invention further provides means to optimize culture conditions for specific subpopulations of cells.
L'invention concerne des procédés et des dispositifs pour la découvert et l'optimisation fondées sur la connaissance de différences entre des types de cellules. L'invention concerne notamment la microscopie optique à asservissement visuel ainsi que des procédés d'analyse. L'invention concerne également des dispositifs permettant de surveiller étroitement dans le temps des centaines de cellules vivantes ; la quantification de réponses physiologiques dynamiques dans des canaux multiples ; la segmentation et l'analyse d'images numériques en temps réel ; le stress cellulaire et la stimulation cellulaire intelligentes, répétitives et appliquées par ordinateur ; enfin la capacité de retourner au même champ de cellules pour des études et une observation à long terme. L'invention concerne en outre un dispositif permettant d'optimiser les conditions de culture pour des sous-populations cellulaires spécifiques.
Callahan Daniel E.
Parvin Bahram
Sim & Mcburney
The Regents Of The University Of California
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