Visualization method for the direct or indirect detection of...

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G01N 33/58 (2006.01) G01N 33/532 (2006.01) G01N 33/559 (2006.01)

Patent

CA 1253422

JAB 438 ABSTRACT VISUALIZATION METHOD FOR THE DIRECT OR INDIRECT DETECTION OF THE REACTION BETWEEN A SPECIFIC BINDING AGENT AND THE CORRESPONDING ACCEPTOR SUBSTANCE IN BLOT OVERLAY ASSAYS A method for detecting and/or determining an agglomerate formed by the reaction between a specific binding agent and the corresponding acceptor substance according to the general methodology of blot overlay assays by using colloidal metal particles labelled components which may be visualized as a coloured signal at the surface of the blotting medium characteristic for the colloidal metal particles used or quantitatively determined following art-known spectophotometric procedures such as densitometry.

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