Vitro processing of fusion proteins

Details Vitro processing of fusion proteins Vitro processing of fusion proteins

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/195 (2006.01) C07K 14/585 (2006.01) C07K 14/65 (2006.01) C07K 14/815 (2006.01) C07K 19/00 (2006.01) C12N 9/60 (2006.01) C12P 21/00 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2047119

There is provided a novel method for the production of a biologically active protein comprising treating a fusion protein consisting of 1, one or multiple successive protein segment(s) each consisting of said biologically active protein the C-terminal amino acid of which is joined to a linker polypeptide sequence L and 2. a polypeptide tag joined to the C-terminal amino acid of said successive protein segment(s), or consisting of 1. a polypeptide tag joined to the N-terminal amino acid of 2. multiple successive protein segments each consisting of a linker polypeptide sequence L and said biologically active protein, with soluble yeast endoprotcase yscF and with soluble yeast carboxypeptidase ysc.alpha., and isolating said biologically active protein.

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