Walk-through mutagenesis

Details Walk-through mutagenesis Walk-through mutagenesis

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 16/18 (2006.01) C12N 9/00 (2006.01) C12N 9/50 (2006.01) C12N 15/13 (2006.01) C12N 15/57 (2006.01) C12P 21/08 (2006.01) C12Q 1/00 (2006.01) C12Q 1/37 (2006.01) G01N 33/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2079802

A method of mutagenesis by which a predetermined amino acid is introduced into each and every position of a selected set of positions in a preselected region (or several different regions) of a protein to produce library of mutants. The method is based on the premise that certain amino acids play crucial role in the structure and function of proteins. Libraries can be generated which contain a high proportion of the desired mutants and are of reasonable size for screening. These libraries can be used to study the role of specific amino acids in protein structure and function and to develop new or improved proteins and polypep- tides such as enzymes, antibodies, single chain antibodies and catalytic antibodies.

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