G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/53 (2006.01) C07C 243/38 (2006.01) C12Q 1/28 (2006.01) G01N 33/531 (2006.01) G01N 33/535 (2006.01)
Patent
CA 2077016
WASH COMPOSITION (CONTAINING SIGNAL STOP REAGENT, TEST KIT AND METHOD OF USE WITH PEROXIDASE-LABELED SPECIFIC BINDING LIGAND Abstract of the Disclosure A buffered composition containing a nonionic or anionic surfactant, and a certain amount of a hydroxamic acid (or salt thereof) or an acyl hydrazine can be used as a wash composition in specific binding assays. Such assays include the use of a peroxidase labeled specific binding reactant to form a detectable peroxidase-labeled specific binding complex. The buffered composition and labeled reactant can be supplied in a diagnostic test kit. The hydroxamic acid or acyl hydrazine can be represented by the structure (I): R-CO-NH-R' wherein R is aryl or 6 to 10 carbon atoms in the aromatic nucleus, alkyl of 1 to 7 carbon atoms, cycloalkyl of 5 to 10 carbon atoms in the ring, or heterocyclyl of 5 to 10 atoms in the ring, at least one of which is an oxygen, nitrogen or sulfur atom, and R' is hydroxy or amino.
Jones Douglas Ronald
Kissel Thomas Robert
Snodgrass Gary Louis
Sprague Lisa Dumers
Warren Harold Chester III
Eastman Kodak Company
Gowling Lafleur Henderson Llp
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