Washing a slide at different locations

Details Washing a slide at different locations Washing a slide at different locations

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2176660

An improved method of separating bound labeled indicator from free labeled indicator in a layer of a test element for immunoassay, comprising a) depositing sample containing a target immunoanalyte onto an exterior surface of a test element in the presence of the labeled indicator, and b) adding wash liquid to the exterior surface by i) depositing a first amount of wash liquid at a first location on the surface in a pool, ii) allowing a portion of the pool to spread out through a first portion of the test element to wash out free labeled indicator, and iii) depositing a second amount of wash liquid at a second location on the surface, to spread out through a second portion of the test element, so that background signal due to non-separated free labeled indicator remaining in the volume is reduced.

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