Water-insoluble glucan from streptococcus

Details Water-insoluble glucan from streptococcus Water-insoluble glucan from streptococcus

C - Chemistry, Metallurgy

12

P

195/140, 260/225

C12P 19/00 (2006.01) C08B 37/00 (2006.01) C12P 19/04 (2006.01)

Patent

CA 1085329

ABSTRACT OF THE DISCLOSURE: A culture medium composed of carbon source, nitrogen source and minerals was sterilized at 120°C for 15 minutes, inoculated strains of the genus Streptococcus and then cultivated at 35°C for 72 hours under aeration and agitation. Subsequent of the cultivation, the resultant was subjected to water- washing and sieving to obtain crude glucan. The crude glucan was then dissolved in NaOH solution and centrifuged. The recovered filtrate was neutralized with HCl and then the insolubilized glucan was collected by centrifugation. The collected glucan was washed with water thoroughly until no chloride ion was detected in the waste water and then dried to form white puritied glucan powder.

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