C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
195/1.12, 150/8.
C07H 21/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2031659
-0- WATER-INSOLUBLE REAGENT, NUCLEIC ACID PROBE, TEST KIT AND DIAGNOSTIC AND PURIFICATION METHODS Abstract of the Disclosure An oligonucleotide is linked to a particle through a protein or carbohydrate to form a water-insoluble nucleic acid probe. The protein or carbohydrate has a pI of about 6 or less, and has been chemically modified with an acylating, alkylating or sulfonylating agent. The particle surface is substantially free of other proteins or carbohydrates. The probe is useful in various diagnostic and purification methods wherein hybridization of the oligonucleotide with a target nucleic acid is possible. In one instance, the probe can be used to capture a DNA strand which has been amplified using polymerase chain reaction techniques.
Findlay John B.
King Marlene M.
Warren Harold C. III
Eastman Kodak Company
Findlay John B.
Gowling Lafleur Henderson Llp
King Marlene M.
Warren Harold C. III
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