C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/02 (2006.01) C07K 17/02 (2006.01) C08B 11/187 (2006.01) C08B 31/12 (2006.01) C08B 37/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 27/327 (2006.01) G01N 33/537 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2112992
Water-soluble reagents and conjugates which are particularly well suited for use, for example, in biologically relevant de- tection, quantification and targetting procedures, e.g. in the fields of immunohistochemistry, detection of immunoreactive species, antibody immobilisation, separation or purification; DNA hybridization tests and flow cytometry, are based on a polymeric carri- er molecule having moderate to high molecular weight and to which are covalently attached one or more moieties derived from divinyl sulfone, each of which moieties is attached to the carrier molecule via a covalent linkage formed between one of the two vinyl groups of a divinyl sulfone molecule and a reactive functionality an the polymeric carrier molecule. In reagents of the inven- tion, at least one such attached moiety in its attached state has the remaining vinyl group free and capable of reaction with a mo- lecular species having a functional group, e.g. a hydroxy group or a thiol group, which is reactive towards the free vinyl group. Such a reaction results in the covalent attachment (conjugation) of the molecular species to the polymeric carrier molecule via a linking group derived from divinyl sulfone, the resulting conjugate being an example of a conjugate of the invention. Preferred water-soluble carrier molecules are polysaccharides, particularly dextrans. The molecular species, of which more than one type may be attached to a carrier molecule, may, for example, be selected from antigens, antibodies, haptens, gene probes, hormones, enzymes, drugs, dyes, fluorophores and radioactively labelled substances. Methods are provided for the preparation of reagents and conjugates of the invention.
Boenisch Thomas
Lihme Allan O. F.
Dako Denmark A/s
Deeth Williams Wall Llp
Immunodex K/s
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