Water-soluble rhodamine dyes and conjugates thereof

Details Water-soluble rhodamine dyes and conjugates thereof Water-soluble rhodamine dyes and conjugates thereof

C - Chemistry, Metallurgy

09

B

C09B 11/24 (2006.01) C07D 311/80 (2006.01) C07D 491/04 (2006.01) C07D 491/06 (2006.01) C07H 19/06 (2006.01) C07H 19/10 (2006.01) C07H 19/16 (2006.01) C07H 19/20 (2006.01) C07H 19/207 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2358923

The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy- transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing. An exemplary rhodamine dye has the following structure: (see above formula)

L'invention concerne de nouveaux colorants de rhodamine solubles dans l'eau à fluorescence rouge et des paires de colorants à transfert d'énergie et fluorescence rouge, ainsi que des conjugués marqués contenant ceux-ci et des procédés d'utilisation de ceux-ci. Les colorants, les paires de colorants à transfert d'énergie et les conjugués marqués sont utiles dans diverses applications à base d'eau, en particulier des techniques impliquant une coloration de cellules, une liaison de protéine, et/ou l'analyse d'acides nucléiques, telles que des techniques d'hybridation et le séquençage d'acides nucléiques.

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