Water-soluble tetrazolium compounds

C - Chemistry – Metallurgy – 07 – D

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C07D 257/04 (2006.01) C12Q 1/32 (2006.01)

Patent

CA 2532211

[PROBLEMS] To provide water-soluble tetrazolium compounds which can form formazanes absorbing long-wavelength rays and are excellent in long-term stability in an aqueous solution and which are favorable for the determination of dehydrogenases or enzyme substrates. [MEANS FOR SOLVING PROBLEMS] Water- soluble tetrazolium compounds represented by the general formula (1): (1) wherein R1 to R19 are each independently hydrogen, nitro, sulfo, or an alkyl, alkoxy, sulfoalkyl, or sulfoalkyloxy group having 1 to 4 carbon atoms, with the proviso that at least two of R1 to R19 are each independently sulfo or a sulfoalkyl or sulfoalkyloxy group having 1 to 4 carbon atoms; and M is alkali metal or ammonium.

[PROBLEME] Obtenir des composés tétrazolium hydrosolubles permettant de former des formazanes absorbant des rayons de grande longueur d'ondes et présentant une excellente stabilité à long terme dans un solution aqueuse, et qui sont avantageux pour déterminer des déshydrogénases ou des substrats enzymatiques. [MOYENS PERMETTANT DE RESOUDRE LE PROBLEME] L'invention concerne des composés tétrazolium hydrosolubles représentés par la formule générale (1): (1) dans cette formule, R1 à R19 sont, indépendamment les uns des autres, hydrogène, nitro, sulfo, ou un groupe alkyle, alkoxy, sulfoalkyle, ou sulfoalkyloxy présentant de 1 à 4 atomes de carbone, pour autant qu'au moins deux des éléments de R1 à R19 représentent, indépendamment l'un de l'autre, sulfo ou un groupe sulfoalkyl ou sulfoalkyloxy présentant de 1 à 4 atomes de carbone; et M représente un métal alcalin ou de l'ammonium.

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