Well unit for detecting cell chemotaxis and separating...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/32 (2006.01) B81C 99/00 (2010.01) B01L 3/00 (2006.01) B81B 1/00 (2006.01) C12M 1/34 (2006.01) G01N 15/14 (2006.01) G01N 33/48 (2006.01) G01N 33/49 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2400738

It is intended to provide a well unit to be used in fabricating an apparatus whereby movements of cells based on their own power can be accurately and easily detected in order to detect cell chemotaxis due to a chemotactic factor or inhibition of cell chemotaxis by an inhibitor. Namely, a well unit to be used in an apparatus for detecting cell chemotaxis and separating cells characterized in that a plural number of wells, in which a liquid sample can be contained in a static state, are connected to each other via channels, the channels are provided with banks, and in the upper part of the banks, one or more grooves having a width and/or a depth fit for the size or deformability of cells are formed, or a plane is formed in such a manner that there is a space between the plane and a glass substrate having a depth fit for the size or deformability of the cells.

L'invention vise à mettre au point une unité cellulaire s'utilisant pour fabriquer un dispositif permettant de détecter de manière précise et aisée les mouvements de cellules sur la base de leur propre pouvoir, afin d'identifier la chimiotaxie cellulaire due à un facteur chimiotactique ou à inhiber la chimiotaxie cellulaire par un inhibiteur. A cet effet, il est prévu une unité cupulaire s'utilisant dans un dispositif servant à détecter la chimiotaxie cellulaire et à séparer les cellules, qui se caractérise en qu'elle comprend plusieurs puits pouvant contenir un échantillon liquide à l'état statique et qui sont interconnectés par l'intermédiaire de canaux. Lesdits canaux sont munis de bancs et dans la partie supérieure des bancs, il est prévu une ou plusieurs rainures dont la largeur et/ou la profondeur sont en ajustement avec les dimensions ou la déformabilité de cellules, ou il est prévu un plan formé de manière à créer un espace entre le plan et le substrat de verre dont la profondeur est en ajustement avec les dimensions ou la déformabilité de cellules.

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