Whisker-mediated transformation of plant cell aggregates and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/87 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2746763

Plant cell aggregates and plant tissues can be transformed by elongated, needle-like structures called 'whiskers'. The process comprises the agitation of plant cell aggregates and plant tissues of the plant to be transformed in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated. The process may be applicable to other plant cell aggregates and plant tissues which have not proven easily transformable by other techniques.

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