Whole blood sample preparation for polymerase chain reaction...

Details Whole blood sample preparation for polymerase chain reaction... Whole blood sample preparation for polymerase chain reaction...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 1/06 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01) G01N 33/50 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2199337

Leucocytes can be quickly and selectively separated from erythrocytes by subjecting a whole blood sample to a series of steps including lysing the erythrocytes and washing the remaining leucocytes with a solution containing ammonium chloride and a carboxylic acid or a metal carboxylate. The resulting white blood cells can be readily lysed and subjected to polymerase chain reaction to amplify and detect a target nucleic acid. The test kit useful in practicing the amplification method includes a labeled primer, a PCR reagent and a reagent mixture containing ammonium chloride and a carboxylic acid or a metal carboxylate for sample preparation.

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