Whole cell assay

Details Whole cell assay Whole cell assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2262012

This invention provides a method for determining the presence of specific cell types in a mixed population of cells. This method involves the identification and detection of markers for the target cell types, the preparation of primers specific for the markers, and the use of reverse transcriptase reaction technology to amplify and detect these markers. The method of this invention is sensitive enough to detect the presence of as few as 100 target cells in a cell population. Specific examples of target cells include human chondrocyte cells and human keratinocyte cells.

Procédé de détermination de la présence de types cellulaires spécifiques dans une population cellulaire mixte. Ce procédé consiste à identifier et à détecter des marqueurs pour les types cellulaires cibles, à préparer des amorces spécifiques des marqueurs, et à utiliser la technologie de la réaction à transcriptase inverse dans le but d'amplifier et de détecter ces marqueurs. Ledit procédé est suffisamment sensible pour détecter la présence d'à peine 100 cellules cibles dans une population cellulaire. Les cellules cibles peuvent être des chondrocytes humains et des kératinocytes humains.

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