Wnt pathway stimulation in reprogramming somatic cells

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/071 (2010.01) C12N 5/0735 (2010.01) A61K 35/12 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2698091

The invention provides compositions and methods of use in reprogramming somatic cells. Compositions and methods of the invention are of use, e.g., for generating or modulating (e.g., enhancing) generation of induced pluripotent stem cells by reprogramming somatic cells. The reprogrammed somatic cells are useful for a number of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a pluripotent state and/or enhances the speed and/or efficiency of reprogramming. Certain of the compositions and methods relate to modulating the Wnt pathway.

La présente invention concerne des compositions et des procédés utilisés dans la reprogrammation de cellules somatiques. Les compositions et procédés de la présente invention servent, par exemple, à générer ou à moduler (par exemple, à augmenter) la génération de cellules souches pluripotentes induites par la reprogrammation de cellules somatiques. Les cellules somatiques reprogrammées sont utiles pour un certain nombre d'objectifs, y compris le traitement ou la prévention d'une affection médicale chez un individu. L'invention concerne en outre des procédés d'identification d'un agent qui reprogramme les cellules somatiques vers un état pluripotent et/ou augmente la vitesse et/ou l'efficacité de la reprogrammation. Certaines des compositions et certains des procédés concernent la modulation de la voie Wnt.

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