Wound repair dressings and methods for their preservation

Details Wound repair dressings and methods for their preservation Wound repair dressings and methods for their preservation

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 5/0775 (2010.01) A01N 1/02 (2006.01) A61K 35/36 (2006.01) A61L 27/60 (2006.01) C12N 1/04 (2006.01)

Patent

CA 2204751

Methods and products relating to the preservation of cultured mammalian epithelial or mesenchymal cells are provided. The methods involve the pre- treatment of epithelial cells with a solution containing a cryoprotectant amount of monosaccharides and/or disaccharides. The treated cells then are frozen, dried or freeze-dried in a minimum volume of solution and stored for later use. The invention avoids the use of materials that must be washed away from the preserved tissue prior to application of the tissue to a wound bed. The invention also permits the storage of epithelial tissue in a dry state.

L'invention porte sur des procédés et des produits relatifs à la conservation de cultures de cellules épithéliales ou mésenchymales de mammifères. Ces procédés consistent à prétraiter des cellules épithéliales à l'aide d'une solution contenant une quantité cryoprotectrice de mono et/ou disaccharides, lesdites cellules étant ensuite congelées, séchées ou lyophilisées dans un volume minimal de solution puis stockées en vue de leur utilisation ultérieure. L'invention permet d'éviter l'utilisation de matériaux susceptibles d'être éliminés des tissus avant leur application sur la plaie; elle permet en outre le stockage de tissus épithéliaux à l'état sec.

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