Xanthan-ester and acridan substrates for horseradish peroxidase

Details Xanthan-ester and acridan substrates for horseradish peroxidase Xanthan-ester and acridan substrates for horseradish peroxidase

C - Chemistry, Metallurgy

07

D

C07D 219/04 (2006.01) C07D 219/06 (2006.01) C07D 311/82 (2006.01) C07D 311/84 (2006.01) C07D 335/12 (2006.01) C12N 9/96 (2006.01) C12Q 1/28 (2006.01) G01N 33/533 (2006.01) G01N 33/573 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2342600

Xanthan-esters and acridans are substrates for horseradish peroxidase. These stable, enzymatically cleavable chemiluminescent esters are substrates for horseradish peroxidase which, together with peroxide is among the extensively used enzyme in enzyme-linked detection methods, including immunoassays, oligonucleotide detection and nucleic acid hybridization. The novel compounds are used, together with peroxide, alkali and the peroxidase, to indicate the presence and/or concentration of target compounds. The assays may be enhanced by the use of polymeric quaternary onium enhancement compounds or similar compounds selected to enhance the chemiluminescence emitted.

Les xanthane-esters et les acridanes sont des substrats pour la peroxydase du raifort. Ces esters chimioluminescents, stables et susceptibles d'être coupés par voie enzymatique sont des substrats pour la peroxydase du raifort qui, avec le peroxyde, est l'enzyme le plus largement utilisé dans les méthodes de détection enzymatiques, notamment les analyses immunologiques, la détection d'oligonucléotides et l'hybridation d'acides nucléiques. Ces nouveaux composés sont utilisés, tout comme le peroxyde, les alcalins et la peroxydase, pour indiquer la présence et/ou la concentration de composés cibles. Ces analyses peuvent être améliorées par l'utilisation de composés polymères d'onium quaternaire ou de composés similaires sélectionnés pour renforcer la chimioluminescence émise.

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