C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) A23K 1/165 (2006.01) C12N 9/42 (2006.01) C12N 15/09 (2006.01) C12P 19/04 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2555063
A xylanase gene, denoted xynR8, encoding a novel xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermo-tolerable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the .beta.-1,4- glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.
Gène, désigné xynR8, codant une xylanase nouvelle (XynR8) obtenue à partir de microorganismes non isolés présents dans la panse des ruminants. La séquence du gène xynR8, codant ladite xylanase, est également fournie; l'enzyme est thermotolérante et présente une grande spécificité pour les xylanes ainsi qu'une activité élevée. La transformation d'hôtes microbiens avec le gène xynR8 est décrite. La présente invention concerne également une méthode permettant la dégradation de structures contenant du xylane, laquelle consiste en l'hydrolyse des liens .bêta.-1,4-glycosidiques des xylanes par contact avec la xylanase. La présente invention concerne enfin une composition utilisant ladite méthode d'hydrolyse.
Chen Yo-Chia
Cheng Hsueh-Ling
Cheng Kou-Joan
Borden Ladner Gervais Llp
Chen Yo-Chia
Cheng Kou-Joan
Genozyme Biotech Inc.
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