C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) G06F 19/22 (2011.01) A21D 8/04 (2006.01) A23G 4/14 (2006.01) A23K 1/165 (2006.01) A23L 1/305 (2006.01) A23L 2/66 (2006.01) A61K 38/47 (2006.01) A61L 12/14 (2006.01) C02F 3/00 (2006.01) C07H 21/04 (2006.01) C07K 16/40 (2006.01) C07K 17/00 (2006.01) C07K 19/00 (2006.01) C11D 7/42 (2006.01) C11D 9/40 (2006.01) C12N 5/16 (2006.01) C12N 9/24 (2006.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12P 7/06 (2006.01) C12P 19/00 (2006.01) C12Q 1/34 (2006.01) C12Q 1/68 (2006.01
Patent
CA 2638801
The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-l,4-xylosidic linkages or endo- .beta.-1,4- ghicanase linkages; and/or degrading a linear polysaccharide beta-l ,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
L'invention concerne des enzymes ayant une activité de xylanase, de mannanase et/ou de glucanase, par exemple catalysant l'hydrolyse de ponts .beta.-1,4-xylosidiques internes ou de ponts endo-.beta.-1,4-glucanase ; et/ou dégradant un polysaccharide bêta-1,4-xylane linéaire en xylose. Ainsi, l'invention concerne des méthodes et des procédés de décomposition d'hémicellulose, qui est un composant majeur de la paroi cellulaire des végétaux, y compris des méthodes et des procédés d'hydrolyse d'hémicelluloses dans n'importe quel végétal ou bois ou un produit à base de bois, un déchet de bois, de la pâte à papier, un produit papetier ou un déchet ou sous-produit papetier. L'invention concerne en outre des procédés de conception de nouvelles xylanases, mannanases et/ou glucanases, ainsi que des procédés pour les utiliser. Ces xylanases, mannanases et/ou glucanases ont une activité et une stabilité augmentée à un pH et une température plus élevés.
Beaver Scott W.
Blum David
Chang Hwai
Chang Kristine
Desantis Grace
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Verenium Corporation
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