C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/52 (2006.01) C12N 9/42 (2006.01) C12N 15/09 (2006.01)
Patent
CA 2701117
The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo- .beta.-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
L'invention porte sur des enzymes à activité de xylanase, de mannanase et/ou de glucanase, catalysant par exemple l'hydrolyse de liaisons internes ß-l,4-xylosidiques et de liaisons internes de l'endo- ß-1,4-glucanase, et/ou dégradant le polysaccharide bêta-1,4-xylane en xylose. L'invention porte ainsi sur des méthodes et procédés de rupture de la paroi des cellules végétales dont des méthodes et procédés d'hydrolyse des hémicelluloses de toute plante ou bois ou produit ligneux, ou déchet de bois, pâte à papier, produits de papier, déchets de papier, ou produits dérivés. L'invention porte également sur des méthodes de désignation de nouvelles xylanases, mannanases et/ou glucanases et sur leurs méthodes d'utilisation. Lesdites xylanases, mannanases et/ou glucanases présentent une activité et une stabilité accrues pour des pH et des températures plus élevés.
Dirmeier Reinhard
Gray Kevin A.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Verenium Corporation
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