C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/24 (2006.01) D21C 9/10 (2006.01) D21H 17/00 (2006.01) D21C 5/00 (2006.01)
Patent
CA 2435527
The present invention provides a xylanase, or a modified xylanase enzyme comprising at least one substituted amino acid residue at a position selected from the group consisting of amino acid 11, 116, 118, 144 and 161, the position determined from sequence alignment of the modified xylanase with Trichoderma reesei xylanase II amino acid sequence. The xylanases described herein exhibit improved thermophilicity, alkalophilicity, expression efficiency, or a combination thereof, in comparison to a corresponding native xylanase.
L'invention concerne une xylanase ou une enzyme xylanase modifiée comprenant au moins un résidu amino-acide substitué au niveau d'une position sélectionnée dans le groupe comprenant l'amino-acide 11, 116, 118, 144 et 161, cette position étant déterminée à partir de l'alignement séquentiel de la xylanase modifiée avec une séquence amino-acide de xylanase II de Trichoderma reesei. Les xylanases selon l'invention présentent un caractère thermophile accru, un caractère alcalophile renforcé, une meilleure efficacité d'expression ou plusieurs de ces propriétés, comparé à une xylane non dénaturée correspondante.
Gowling Lafleur Henderson Llp
National Research Council Of Canada
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