C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/22 (2006.01) C12P 7/06 (2006.01) C12P 7/10 (2006.01)
Patent
CA 2661512
A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism.lt also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.
Selon la présente invention, une souche de zymomonase utilisant le xylose a été obtenue à la suite d'une modification génétique du gène d'oxydoréductase glucose-fructose, ce qui a permis de réduire l'expression de l'activité de l'enzyme GFOR. La souche produit moins de xylitol, un produit dérivé nocif provenant du métabolisme du xylose. De plus, cette souche consomme plus de xylose et produit plus d'éthanol au cours de la fermentation mixte du sucre dans des conditions de traitement adéquates.
Chou Yat-Chen
Mccutchen Carol M.
Viitanen Paul V.
Zhang Min
Alliance For Sustainable Energy Llc
E. I. Du Pont de Nemours And Company
Torys Llp
LandOfFree
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