Y-glutamyltranspeptidase, its preparation and its use

Details Y-glutamyltranspeptidase, its preparation and its use Y-glutamyltranspeptidase, its preparation and its use

C - Chemistry, Metallurgy

12

N

195/123, 195/33.

C12N 9/10 (2006.01) C12P 35/02 (2006.01)

Patent

CA 1340504

The invention relates to substantially purified .gamma.-glutamyltranspeptidase (.gamma.-GTP) having the following characteristics: - a molecular weight of 23,000 to 40,000 Dalton - a isoelectric point at a pH between 5.0 and 6.5, - a pH optimum of 6.5 to 10 with L-.gamma.-glutamylparanitroanilide as substrate - a K m of 9 to 36 µM at pH 8, and - the ability to hydrolyze glutaryl- or adipinyl-monoamino compounds - obtainable by fermentation of bacteria of one or more of the genus Pseudomonas, Proteus, Arthrobacter and Bacillus.

556683

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