C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/19 (2006.01) C12N 1/16 (2006.01) C12N 15/09 (2006.01) C12N 15/81 (2006.01) C12P 1/02 (2006.01) C12P 7/06 (2006.01) C12P 7/56 (2006.01) C12N 9/04 (2006.01) C12N 15/53 (2006.01)
Patent
CA 2645361
Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluy.upsilon.eromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.
Le procédé selon l'invention consiste à modifier génétiquement des cellules de levure pour dissocier une voie métabolique native de dihydroxyacétone en glycérol. Selon certains aspects, le cellule de levure appartient au genre Kluy.upsilon.eromyces, Candida ou Issatchenkia. Dans d'autres aspects, la cellule de levure est capable de produire au moins un acide organique, tel que du lactate. Les cellules de levure produisent considérablement moins de glycérol que les souches de type sauvage et permettent d'obtenir généralement des rendements supérieurs de produits de fermentation désirés. Les cellules de levure selon l'invention ont une croissance satisfaisante lorsqu'elles sont cultivées, et ce, malgré leur production restreinte en glycérol.
Aristidou Aristos
Dundon Catherine Asleson
Hause Benjamin Matthew
Koivuranta Kari
Mcmullin Thomas William
Cargill Inc.
Smart & Biggar
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