Yeast host strains with defects in n-glycosylation

Details Yeast host strains with defects in n-glycosylation Yeast host strains with defects in n-glycosylation

C - Chemistry, Metallurgy

12

N

C12N 1/18 (2006.01) C12N 1/16 (2006.01) C12N 1/19 (2006.01) C12N 9/04 (2006.01) C12N 15/01 (2006.01) C12N 15/81 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2103522

Saccharomyces mutants with defects in N-glycosylation which are obtainable by [3H]-mannose suicide selection, introduction of one or several selective markers, selection of those strains which, after transformation with the plasmid YEpL/GOD, secrete 10 mg/l GOD or more into the culture medium after culture under standard conditions, are allelic to the ngd mutations in Saccharomyces cerevisiae, DSM 7042, DSM 7338, DSM 7160 and/or 7340 and express proteins with a uniform carbohydrate structure.

Saccharomyces mutants déficients à l'égard de la N-glycosylation que l'on peut obtenir par sélection suicidaire au [3H]-mannose, introduction d'un ou de plusieurs marqueurs de sélection, sélection des souches qui, après transformation par le plasmide YEpL/GOD, sécrètent 10 mg/L de glucose oxydase ou plus dans le milieu de culture après culture dans des conditions standard, sont alléliques pour ce qui est des mutations ngd chez Saccharomyces cerevisiae, DSM 7042, DSM 7338, DSM 7160 et/ou 7340 et expriment des protéines ayant une structure glucidique uniforme.

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