Yeast promoter and process for preparing heterologous protein

Details Yeast promoter and process for preparing heterologous protein Yeast promoter and process for preparing heterologous protein

C - Chemistry, Metallurgy

12

N

195/1.24, 195/1.

C12N 15/81 (2006.01) C07K 14/02 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1310602

M-37-4500C/KT/87 ABSTRACT OF THE DISCLOSURE A method of producing a heterologous proteins in yeast is disclosed, which comprises transforming a yeast Saccharomyces cerevisiae with recombinant DNA com- prising a promoter selected from the group consisting of a yeast promoter or a hybrid promoter derived from a yeast promoter, particularly a hybrid promoter containing the enhancer region of SV40 virus, and a gene coding for a heterologous protein such as HBsAg and Pre S-HBsAg, par- ticularly full length Pre S-HBsAg culturing,the resulting transformed cells to express the gene coding the heterolo- gous protein, and isolating the heterologous protein from the cultured medium. GAP-DH and PHO5 promoters and miniaturized ones can be used as the yeast promoter. The expression of the full length Pre S-HBsAg is carried out in the same expression system as that for 2nd or 3rd Pre S-HBsAg.

538634

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