Yeast strains producing cellulolytic enzymes and methods and...

Details Yeast strains producing cellulolytic enzymes and methods and... Yeast strains producing cellulolytic enzymes and methods and...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/56 (2006.01) C12N 1/19 (2006.01) C12N 9/42 (2006.01) C12N 15/62 (2006.01) C12N 15/81 (2006.01)

Patent

CA 1305931

YEAST STRAINS PRODUCING CELLULOLYTIC ENZYMES AND METHODS AND MEANS FOR CONSTRUCTING THEM ABSTRACT OF THE DISCLOSURE Described are methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes. This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expres- sion of these cellulolytic genes in yeast leads to the produc- tion of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze .beta.-1,4-glucan substrates such as cellulose.

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