Yeast vector

Details Yeast vector Yeast vector

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/81 (2006.01) C12N 1/19 (2006.01)

Patent

CA 1339099

A 2µm plasmid vector for transforming yeast, especially brewing yeast, comprises a DNA sequence allowing propagation of the plasmid in bacteria, two copies of the 74 base pair FLP recombination site of the 2µm plasmid in direct orientation and a DNA sequence coding for a protein or peptide of interest. The plasmid is so constructed that in yeast the bacterial DNA sequence is spontaneously lost. A "gene of interest" is preferably inserted at the SnaBI site.

563822

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