Yolk sac stem cells and their uses

Details Yolk sac stem cells and their uses Yolk sac stem cells and their uses

A - Human Necessities

01

K

A01K 67/027 (2006.01) A61K 35/54 (2006.01) C12N 5/02 (2006.01) A61K 35/12 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2166694

The present invention is directed to mammalian yolk sac stem cells. In particular, it relates to the characterization, culturing, long-term expansion and uses of yolk sac stem cells for in vivo reconstitution and therapy. Yolk sac stem cells isolated from the early embryonic yolk sac prior to blood island formation exhibit a homogeneous morphology and a primitive cell surface phenotype without the expression of mature leukocyte markers and major histocompatibility complex-encoded antigens. The cells can be cultured and expanded long-term with minimal differentiation, and without alteration of their pluripotency. However, such cells can be induced to express various blood cell markers upon stimulation with specific cytolcines. Yolk sac stem cells may have a wide range of applications including but not limited to the reconstitution of a destroyed or deficient human hematopoietic system, and the construction of large and small animal models for the production of human blood cells, human antibodies, and testing of human diseases, immune function, vaccines, drugs and immunotherapy.

Cellules souches de sac vitellin de mammifère et en particulier caractérisation, culture, expansion à long terme et utilisation de cellules souches de sac vitellin pour la reconstitution in vivo et la thérapie. Les cellules souches de sac vitellin isolées du sac vitellin embryonnaire précoce avant la formation d'îlots sanguins présentent une morphologie homogène et un phénotype de surface cellulaire primitif sans l'expression des marqueurs de leucocytes matures et des antigènes codés par le complexe d'histocompatibilité majeur. Lesdites cellules peuvent être cultivées et multipliées à long terme avec une différenciation minimale et sans altération de leur pluripotence. Toutefois, lesdites cellules peuvent être amenées à exprimer divers marqueurs de cellules sanguines après stimulation à l'aide de cytokines spécifiques. Lesdites cellules souches de sac vitellin peuvent avoir une large plage d'applications, y compris, entre autres, la reconstition d'un système hématopoïétique humain détruit ou déficient et la construction de modèles animaux grands et petits pour la production de cellules sanguines humaines, d'anticorps humains et le test de maladies humaines, de la fonction immune, de vaccins, de médicaments et de l'immunothérapie.

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