Znf206: a novel regulator of embryonic stem cell...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/18 (2006.01) C12N 5/0735 (2010.01) C12N 15/113 (2010.01) A61K 31/7088 (2006.01) A61K 48/00 (2006.01) C07H 21/00 (2006.01) C07K 14/475 (2006.01) C07K 16/22 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2695505

We have identified ZNF206, a novel repressor of human embryonic stem cell (hESC) differentiation. Repressing extra-embryonic endoderm development preserves the pluripotent state of human embryonic stem cells, and, conversely downregulating expression of ZNF206 in hESCs causes them to upregulate the expression of genes associated with the extra-embryonic endodermal lineage, down-regulate genes associated with the pluripotent state, and may lead to the further emergence of genes associated with even more differentiated lineages and phenotypes.

L'invention concerne ZNF206, un nouveau répresseur de la différenciation des cellules souches embryonnaires humaines (hESC). La répression du développement extra-embryonnaire de l'endoderme préserve l'état pluripotent de cellules souches embryonnaires humaines, et, en régulant réciproquement à la baisse l'expression de ZNF206 dans des hESC, provoquant une régulation à la hausse de l'expression de gènes associés à la lignée endodermique extra-embryonnaire, régule à la baisse les gènes associés à l'état pluripotent, et peut aboutir à l'émergence ultérieure de gènes associés à des lignées et des phénotypes encore plus différenciés.

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