Zoogloeal and hyphomicrobium spp. nucleic acids

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2305888

The invention provides two new zoogloeal strains, mzlt and mz2t. The invention provides an isolated nucleic acid consisting of the nucleic acid of SEQ ID NO: 1. Examples of nucleic acids of mz1t include SEQ ID NO: 2 and SEQ ID NO: 3. An example of a nucleic acid of mz2t is SEQ ID NO: 4. The invention also provides an isolated nucleic acid consisting of the nucleic acid of SEQ ID NO: 5. A method of detecting the presence of zoogloeal clusters in a wastewater sample is provided, comprising: a) contacting RNA from a sample of the wastewater with a nucleic acid comprising the nucleic acid of SEQ ID Nos: 1, 2, 3, 4 or 5 under conditions that permit specific hybridization; and b) detecting the presence of hybridization, the presence of hybridization indicating the presence of zoogloeal clusters. A new Hyphomicrobium spp. strain, designated M3, is provided herein. The invention provides novel nucleic acids of a Hyphomicrobium sp. M3. For example SEQ ID NO: 6 and SEQ ID NO: 7 rDNAs of M3. SEQ ID Nos: 8, 9, 10, 11, 12, 13 and 14 are presumptive Hyphomicrobium spp. sequences. SEQ ID NO: 15 is a consensus sequence from 6m3 and 3m3 (Hyphomicrobium sp. m3 isolated from sludge). A method of detecting the presence of a Hyphomicrobium species in a wastewater sample as described above, using a nucleic acid comprising the nucleic acid of SEQ ID NO: 17 is also provided. The nucleic acids of SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15 can also be utilized in whole or as fragments in the detection method for Hyphomicrobium spp.

L'invention concerne deux nouvelles souches de la zooglée, mz1t et mz2t, ainsi qu'un acide nucléique renfermant l'acide nucléique de SEQ ID NO:1. Des exemples d'acides nucléiques de mz1t sont SEQ ID NO:2 et SEQ ID NO:3. Un exemple d'acide nucléique de mz2t est SEQ ID NO:4. L'invention concerne un acide nucléique renfermant la séquence SEQ ID NO:5. Elle concerne une technique permettant de détecter la présence d'amas de zooglée dans un échantillon d'eau usée, selon laquelle: a) on met en contact l'ARN extrait d'un échantillon d'eau usée avec un acide nucléique renfermant l'acide nucléique de SEQ ID NO:1, 2, 3, 4 ou 5, dans des conditions qui permettent une hybridation spécifique; et b) on détecte la présence de l'hybridation, celle-ci indiquant la présence d'amas de zooglée. L'invention concerne une nouvelle souche de Hyphomicrobium spp., désignée par M3, ainsi que de nouveaux acides nucléiques d'une souche M3 de Hyphomicrobium spp., par exemple les ADNr SEQ ID NO:6 et SEQ ID NO:7 de M3. SEQ ID NO:8, 9, 10, 11, 12, 13 et 14 sont des séquences présumées de Hyphomicrobium spp. SEQ ID NO:15 est une séquence consensus extraite de 6m3 et 3m3 (Hyphomicrobium sp. m3 isolé de la boue). L'invention concerne une technique permettant de détecter la présence d'une espèce de Hyphomicrobium dans un échantillon d'eau usée, similaire à technique décrite ci-dessus, au moyen d'un acide nucléique renfermant l'acide nucléique de SEQ ID NO:17. Les acides nucléiques de SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 et SEQ ID NO:15 peuvent également être utilisés en totalité ou sous forme de fragments dans la technique de détection de Hyphomicrobium spp.

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