Zymogenic nucleic acid detection methods, and related...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2321948

This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.

L'invention concerne des méthodes de détection et d'évaluation quantitative d'une séquence d'acide nucléique cible dans un échantillon. Ces méthodes consistent à mettre l'échantillon en contact avec une amorce et un zymogène qui code pour une séquence d'acide nucléique, mais dont il est lui-même la séquence antisens, de manière que, lorsque la cible est présente, une seule molécule d'acide nucléique amplifié est produite, laquelle comprend les séquences de la cible et des molécules catalytiques. L'invention concerne également une méthode de détection simultanée d'une pluralité de séquences d'acide nucléique cible dans un échantillon. Enfin, l'invention concerne des molécules et des trousses pour mettre en pratique lesdites méthodes.

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