Zymomonas with improved ethanol production in medium...

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 7/06 (2006.01)

Patent

CA 2704539

Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of thehimA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.

Selon l'invention, par un criblage d'une bibliothèque de Zymomonasmutant, le gène himA a été trouvé être mis en jeu dans l'effet inhibiteur de l'acétate sur la performance de Zymomonas. Des souches de Zymomonas utilisant du xylose davantage synthétisées pour réduire l'activité du gène himA ont été trouvées avoir une production d'éthanol accrue par comparaison avec une souche parentale, lorsqu'elles sont cultivées dans un milieu à sucres mélangés comprenant du xylose et, en particulier, en présence d'acétate.

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