Zymomonas with improved xylose utilization

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) C12N 9/92 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2716585

Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

Selon l'invention, des souches de Zymomonas ont été mises au point par introduction d'un gène xylose isomérase chimérique qui contient un promoteur mutant du gène Z. mobilis glycéraldéhyde-3-phosphate déhydrogénase. Le promoteur oriente une expression accrue de xylose isomérase, et lorsque la souche est en plus mise au point pour l'expression de xylulokinase, transaldolase et transcétolase, on obtient une utilisation améliorée de xylose.

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