Process for preparing artificial red cells

Details Process for preparing artificial red cells Process for preparing artificial red cells

A - Human Necessities

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A61K 35/14 (2006.01) A61K 9/50 (2006.01) A61K 9/64 (2006.01) A61K 35/18 (2006.01) B01J 13/02 (2006.01) C07K 14/805 (2006.01) A61K 38/00 (2006.01) A61M 1/36 (2006.01)

Patent

CA 1162849

ABSTRACT OF THE DISCLOSURE A process for preparing artificial red cells comprising microdroplets of aqueous, stroma-free hemo- globin solution encapsulated in membranes of polymerized hemoglobin, said process comprising forming microdroplets of hemoglobin solution in a continuous oil phase and cross-linking the hemoglobin at the surface of the micro- droplets with a suitable oil-soluble cross-linking agent to form the artificial red cells and recovering the cells. The oil-soluble cross-linking agent is present in the oil phase containing the hemoglobin microdroplets and the red cells are removed from the oil phase by contacting same with an aqueous suspending phase containing a sur- factant having an HLB of at least 9.

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